Genome Sciences Centre

AcePrimer 1.3.2 -- PCR primers for the C. elegans genome

Design Parameters		Target Region OR Target Gene
		WormBase Version
Primer sets (per exon if Gene selected)		Chromosome	Primers flanking region from to
Product size (bp)	+/-	Gene (Info)	Target exon
Primer size	+/-	Poison and Nested Primers
Optimum Tm (C)	+/-	Poison primers	Separation bp
GC clamp (bp)		Nested primers	Interval bp
Maximum Q. value		RT-PCR Primers
Allow primers in nearby genes		Minimum coding DNA bp
		e-PCR Options
		Word size Mismatches