Genome Sciences Centre - PCR Primer Design for C. elegans promoter-GFP Fusions

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Design Parameters		Optional
WormBase Version
Gene		GC clamp	bp.
Product size	+/-	Max. Q value
Number of primer sets		Target upstream from	to bp.
Primer size	+/-	e.g. To target a 2kb region 1kb from the ATG start enter "1000" to "3000." This over-rides Product Size.
Optimum Tm	+/-	Allow primers in upstream genes
		Exclude tandem repeats

Nested Primers		e-PCR Options
Number of nested sets		Word size
Primer separation	bp	Allowed mismatches
		Run e-PCR check (Slower but recommended)